MicroRNA‐296‐5p promotes healing of diabetic wound by targeting sodium‐glucose transporter 2 (SGLT2)

Abstract Background Diabetic wounds are refractory and very difficult to heal. We aimed to use miRNA to identify novel and specific molecular markers for diabetes mellitus (DM) diagnosis and treatment. Methods The expression level of miR‐296‐5p was determined in tissue samples of 12 DM patients. The effect of miR‐296‐5p on proliferation of β‐cells was examined using Cell Counting Kit‐8 (CCK‐8) and colony formation assay. The effect of miR‐296‐5p on cell cycle progression was analysed using flow cytometry. The target gene was verified using luciferase reporter assay. A rat diabetes model was used to assess the effect of miR‐296‐5p in vivo . Results Overexpression of miR‐296‐5p suppressed cell proliferation, arrested cell cycle progression, and increased the healing rate of diabetic wounds both in vivo and in vitro . TargetScan analysis results showed that miR‐296‐5p is a direct regulator of SGLT2 . Conclusions miR‐296‐5p can increase the healing rate of diabetic wounds and may be an effective molecular tool in DM diagnosis and therapy.