内吞作用
转铁蛋白
赫拉
转铁蛋白受体
共轭体系
受体介导的内吞作用
化学
生物物理学
细胞生物学
肽
纳米技术
细胞
材料科学
生物化学
生物
有机化学
聚合物
作者
Siying Li,Ruixia Wang,Junfeng Li,Yulin Liu,Yanfeng Fu,Jing Zhou,Guocheng Yang,Yuping Shan
标识
DOI:10.1021/acs.molpharmaceut.0c01119
摘要
The HAIYPRH (T7) peptide has been widely used as a ligand for constructing tumor-targeted nanodrug delivery systems since it can target the transferrin receptor (TfR) and then enter cells easily with the help of transferrin (Tf). However, the dynamic mechanism by which transferrin promotes the entry of T7-conjugated nanostructures into cells remains unclear. Herein, a force tracing technique based on atomic force microscopy (AFM) was used to track the ultrafast dynamic process of a T7-conjugated gold nanoparticle (AuNP-T7) entering a cell at the single-particle level in real time. Tf helped decrease the endocytosis force and increase the endocytosis speed of AuNP-T7 in A549 cells. However, Tf only increased the endocytosis speed of AuNP-T7 in HeLa cells. In contrast, in Vero cells without TfR overexpression, Tf decreased the endocytosis speed. This report provides important insights for redesigning and developing T7-conjugated nanodrug carriers in targeted nanodrug delivery systems.
科研通智能强力驱动
Strongly Powered by AbleSci AI