诱导多能干细胞
细胞生物学
生物
体外
胚胎干细胞
重编程
干细胞
胚状体
再生医学
类有机物
作者
Young Hwang,Shinnosuke Suzuki,Yasunari Seita,Jumpei Ito,Yuka Sakata,Hirofumi Aso,Kei Sato,Brian P. Hermann,Kotaro Sasaki
标识
DOI:10.1101/2020.09.08.286930
摘要
ABSTRACT Establishment of spermatogonia throughout the fetal and postnatal period is essential for production of spermatozoa and male fertility. Here, we established a protocol for in vitro reconstitution of human prospermatogonial specification whereby human primordial germ cell (PGC)-like cells (hPGCLCs) differentiated from human induced pluripotent stem cells were further induced into M-prospermatogonia-like cells (MLCs) and T1 prospermatogonia-like cells (T1LCs) using long-term cultured xenogeneic reconstituted testes. Single cell RNA-sequencing was used to delineate the lineage trajectory leading to T1LCs, which closely resemble human T1-prospermatogonia in vivo and exhibited gene expression related to spermatogenesis and diminished proliferation, a hallmark of quiescent T1 prospermatogonia. Notably, this system enabled us to visualize the dynamic and stage-specific regulation of transposable elements during human prospermatogonial specification. Together, our findings pave the way for understanding and reconstructing human male germline development in vitro.
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