褐背天牛
免疫原性
海胆
免疫系统
化学
生物物理学
细胞生物学
细胞外
胶体金
吞噬作用
纳米毒理学
紫圆线虫
生物化学
生物
纳米技术
纳米颗粒
免疫学
材料科学
作者
Andi Alijagić,Francesco Barbero,Daniela Gaglio,Elisabetta Napodano,Oldřích Benada,Olga Kofroňová,Víctor Puntes,Neus G. Bastús,Annalisa Pinsino
标识
DOI:10.1016/j.jhazmat.2020.123793
摘要
We report that the immunogenicity of colloidal gold nanoparticles coated with polyvinylpyrrolidone (PVP–AuNPs) in a model organism, the sea urchin Paracentrotus lividus, can function as a proxy for humans for in vitro immunological studies. To profile the immune recognition and interaction from exposure to PVP–AuNPs (1 and 10 μg mL−1), we applied an extensive nano-scale approach, including particle physicochemical characterisation involving immunology, cellular biology, and metabolomics. The interaction between PVP–AuNPs and soluble proteins of the sea urchin physiological coelomic fluid (blood equivalent) results in the formation of a protein “corona” surrounding the NPs from three major proteins that influence the hydrodynamic size and colloidal stability of the particle. At the lower concentration of PVP–AuNPs, the P. lividus phagocytes show a broad metabolic plasticity based on the biosynthesis of metabolites mediating inflammation and phagocytosis. At the higher concentration of PVP–AuNPs, phagocytes activate an immunological response involving Toll-like receptor 4 (TLR4) signalling pathway at 24 hours of exposure. These results emphasise that exposure to PVP–AuNPs drives inflammatory signalling by the phagocytes and the resolution at both the low and high concentrations of the PVP–AuNPs and provides more details regarding the immunogenicity of these NPs.
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