光遗传学
细胞生物学
细胞骨架
生物物理学
化学
HEK 293细胞
表位
生物
细胞
生物化学
抗体
受体
免疫学
神经科学
作者
Sangkyu Lee,Hye Rim Park,Taeyoon Kyung,Na Yeon Kim,Sung‐Soo Kim,Jihoon Kim,Won Do Heo
出处
期刊:Nature Methods
[Springer Nature]
日期:2014-05-04
卷期号:11 (6): 633-636
被引量:183
摘要
We present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP.
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