代谢组学
脐静脉
代谢组
三羧酸
糖酵解
纳米片
柠檬酸循环
光热治疗
细胞凋亡
脂质代谢
材料科学
化学
细胞生物学
生物物理学
生物
新陈代谢
生物化学
纳米技术
生物信息学
体外
作者
Dingkun Zhang,Wen Zheng,Xin Li,Ang Li,Nan Ye,Lu Zhang,Yueqiu Liu,Xin Liu,Rui Zhang,Ming Wang,Jun Cheng,Hao Yang,Meng Gong
出处
期刊:Carbon
[Elsevier]
日期:2021-06-01
卷期号:178: 810-821
被引量:23
标识
DOI:10.1016/j.carbon.2021.04.023
摘要
Ti3C2 is the first member of two-dimensional (2D) transition metal carbide or carbonitride (MXene) with excellent photothermal effect in disease treatment on an animal model, which shows a potential prospect on clinical application. However, in-depth details on the biosafety of MXene were insufficient. Metabolomics approach has offered an excellent alternative with numerous bioinformation, which can demonstrate the response of an organism to external stimuli at the molecular level. In this study, two different concentrations (100 and 500 mg/L) of MXene were used to treat human umbilical vein endothelial cells (HUVECs), and under both conditions, no obvious acute cytotoxicity was observed by cell proliferation and apoptosis measurement. We used a platform combining LC-MS/MS, GC-MS, and bioinformatics to analyze the changes of HUVECs metabolome after treatment with different concentrations of MXene, and the results indicated that —high concentrations (500 mg/L) of MXene can cause significant changes in the energy metabolism of HUVECs, showing obvious inhibition of tricarboxylic acid (TCA) cycle with the enhancement of glycolysis, fatty acid biosynthesis, and lipid accumulation, which are closely related to the functional disorder of mitochondria. The metabolomics results of the novel MXene-cell system extend the knowledge on the biological effect of MXene, possibly enabling technological innovations and material modifications.
科研通智能强力驱动
Strongly Powered by AbleSci AI