纤维素酶
矿化(土壤科学)
介孔材料
吸附
多孔性
固定化酶
基质(水族馆)
化学
漆酶
酶
色谱法
化学工程
材料科学
催化作用
生物化学
有机化学
工程类
氮气
海洋学
地质学
作者
Jiacong Wu,Juan Han,Yanli Mao,Lei Wang,Yi Wang,Yuanyuan Li,Yun Wang
标识
DOI:10.1016/j.seppur.2022.121505
摘要
A metal–organic frameworks (MOFs) with adjustable mesopores was prepared through bionic mineralization using protein as template in this paper. By regulating the content of bovine serum, pore structures of different sizes (6.46, 7.55, 10.80 nm) were obtained, used for the immobilization of enzymes (HRP, laccase and cellulase). It was found that the UIO-66 with a pore size of 6.46 nm exhibited the highest loading capacities for the investigated three enzymes. Under optimized conditions for immobilization, the maximum loading capacity of HRP, laccase and cellulase was 205.7, 214.2 and 203.9 mg g−1, respectively. It should be noted that the stabilities of the three immobilized [email protected], [email protected] and [email protected] were significantly improved due to the favorable fitting between the pore size of the carrier material and the enzyme molecular size. In particular, the substrate affinity of the immobilized [email protected] (0.120 g L−1) is similar to that of free HRP (0.122 g L−1). It is mainly due to that the appropriate porous structure of the carrier could avoid the obstruction of the substrate mass transfer channel after the adsorption of enzyme molecules. Moreover, the porous structure has a certain enrichment effect on the substrate, which promotes the catalytic efficiency of immobilized enzymes.
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