免疫分析
医学
抗体
抗核抗体
皮肌炎
内科学
卡帕
自身抗体
胃肠病学
免疫学
语言学
哲学
作者
Grace Skemp-Dymond,Dorota Lebiedz-Odrobina,Lauren M. Zuromski,Jamie L W Rhoads,Anne E. Tebo
出处
期刊:American Journal of Clinical Pathology
[Oxford University Press]
日期:2021-10-18
卷期号:157 (4): 506-509
被引量:1
摘要
To evaluate the performance characteristics of a line immunoassay (LIA) for the detection of Mi-2 antibodies associated with dermatomyositis (DM).In total, 432 consecutive patient specimens were tested for Mi-2 antibodies concurrently by LIA (Mi-2α or Mi-2β) or immunoprecipitation (IP) test and antinuclear antibody by indirect immunofluorescence assay using HEp-2 substrate. Following antibody evaluation, results for patients positive in any of the assays for Mi-2 antibody had a retrospective chart review for diagnostic categorization. The performance of all tests was evaluated based on the extracted clinical data.Forty patients were positive in at least one of the Mi-2 assays. The frequency of Mi-2β antibody by LIA was highest (75.0%), followed by Mi-2 by IP (35.0%) and Mi-2α by LIA (20.0%), respectively. Mi-2 by IP had the best total percent agreement for DM (95.0%) compared with 70.0% and 25.0% for the LIA Mi-2α and Mi-2β, respectively. Positivity of the Mi-2β antibody was significantly associated with non-DM diagnosis.Agreement for DM with assays for detecting Mi-2 is variable. Additional studies are required to validate Mi-2 immunoassays for routine patient evaluation.
科研通智能强力驱动
Strongly Powered by AbleSci AI