pH and ultrasound dual-responsive drug delivery system based on PEG–folate-functionalized Iron-based metal–organic framework for targeted doxorubicin delivery

热重分析 阿霉素 药物输送 傅里叶变换红外光谱 核化学 动态光散射 纳米颗粒 材料科学 PEG比率 结合 对偶(语法数字) 热稳定性 分散性 组合化学 化学 盐酸阿霉素 前药 聚乙二醇 靶向给药 胶束 药品 金属有机骨架
作者
Ahmed H.S Ahmed,Abdollah Karami,Rana Sabouni,Ghaleb A. Husseini,Vinod Paul
出处
期刊:Colloids and Surfaces A: Physicochemical and Engineering Aspects [Elsevier]
卷期号:626: 127062-127062 被引量:7
标识
DOI:10.1016/j.colsurfa.2021.127062
摘要

In recent years, the use of metal–organic frameworks (MOFs) as drug nanocarriers has gained attention because of their extraordinary physical and chemical properties. In this work, dual-responsive iron-based MOFs were synthesized via the microwave-assisted method using FeCl 3 .6 (H 2 O) as the metal cluster and 2-aminoterephthalic acid (NH 2 -BDC) as the organic linker (namely NH 2 -Fe-BDC) and loaded with the anti-cancer drug doxorubicin (DOX). The DOX-loaded MOFs were further functionalized with polyethylene glycol-folate (PEG–FA), yielding PEG–FA-NH 2 -Fe-BDC. The folate moiety is used to specifically target several cancers overexpressing the folate receptor (FR). These nanoparticles were characterized using Fourier-Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Thermogravimetric Analysis (TGA), and Dynamic Light Scattering (DLS). The FTIR confirmed the PEG–FA conjugation to the MOFs, while the XRD patterns confirmed the crystallinity of the nanoparticles. TGA results demonstrated the thermal stability of the MOFs. Moreover, the DLS analysis showed that regular MOFs had a particle diameter of 577 nm, while the PEG–FA-functionalized MOF had a particle diameter of 461 nm, which demonstrates the improved colloidal stability of the functionalized MOF. The DOX encapsulation efficiency was determined to be approximately 97%, while the encapsulation capacity was around 14.5 wt%. Furthermore, the in-vitro release profiles were studied under different pH values (5.3 and 7.4) with and without low-frequency ultrasound (LFUS, at 40 kHz). The results confirmed the sonosensitivity of the nanovehicles, with US-triggered release efficiency reaching up to 90% after 280 min (at a pH of 5.3). The MTT study revealed that these nanocarriers are non-toxic at lower concentrations. Their toxicity increases at higher concentrations. Furthermore, the cellular uptake was investigated via flow cytometry, and the results showed that the conjugation of the PEG-FA moiety to the MOF’s surface significantly enhanced uptake by cancer cells. Accordingly, this study showed the pH/US dual-responsive capability of NH 2 -Fe-BDC and PEG–FA-NH 2 -Fe-BDC.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
sioc_ZN完成签到,获得积分10
1秒前
刘官昊发布了新的文献求助10
1秒前
燕子发布了新的文献求助10
2秒前
zz发布了新的文献求助10
2秒前
郭火锅完成签到,获得积分10
2秒前
4秒前
5秒前
美好的胡萝卜关注了科研通微信公众号
6秒前
6秒前
dcc完成签到,获得积分10
6秒前
9秒前
仙女的小可爱完成签到 ,获得积分10
11秒前
12秒前
dcc发布了新的文献求助20
15秒前
燕子完成签到,获得积分20
17秒前
我是老大应助曲书文采纳,获得10
17秒前
赘婿应助lelecha采纳,获得10
17秒前
一杯冰美式完成签到,获得积分10
18秒前
19秒前
仄言发布了新的文献求助10
24秒前
25秒前
852应助快点毕业吧采纳,获得10
25秒前
27秒前
29秒前
hhhh应助落伍的螃蟹采纳,获得10
29秒前
30秒前
Azizt完成签到,获得积分10
31秒前
31秒前
小巧的芷文完成签到,获得积分10
31秒前
重要晓槐完成签到 ,获得积分20
32秒前
可爱的函函应助fff采纳,获得10
35秒前
仄言完成签到,获得积分10
35秒前
墨客完成签到,获得积分20
35秒前
anna1992发布了新的文献求助10
36秒前
37秒前
斯文子骞发布了新的文献求助10
37秒前
38秒前
kyt完成签到,获得积分10
38秒前
nanci发布了新的文献求助10
41秒前
高分求助中
请在求助之前详细阅读求助说明 20000
One Man Talking: Selected Essays of Shao Xunmei, 1929–1939 1000
Yuwu Song, Biographical Dictionary of the People's Republic of China 700
[Lambert-Eaton syndrome without calcium channel autoantibodies] 520
The Three Stars Each: The Astrolabes and Related Texts 500
Sphäroguß als Werkstoff für Behälter zur Beförderung, Zwischen- und Endlagerung radioaktiver Stoffe - Untersuchung zu alternativen Eignungsnachweisen: Zusammenfassender Abschlußbericht 500
Revolutions 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2460846
求助须知:如何正确求助?哪些是违规求助? 2130538
关于积分的说明 5428397
捐赠科研通 1857748
什么是DOI,文献DOI怎么找? 923936
版权声明 562463
科研通“疑难数据库(出版商)”最低求助积分说明 494230