肝细胞癌
乙型肝炎病毒
生物
断点
基因组
病毒学
乙型肝炎
插入顺序
计算生物学
遗传学
病毒
基因
染色体
转座因子
作者
Weiyang Li,Wei Wei,Fei Hou,Hanshi Xu,Xiaofang Cui
出处
期刊:Genomics
[Elsevier]
日期:2022-01-01
卷期号:114 (1): 23-30
被引量:4
标识
DOI:10.1016/j.ygeno.2021.11.025
摘要
HBV integration and function has gradually been expanding. However, the exact mode of HBV integration remains unclear. In our research, the high-throughput long-read sequencing was combined with bioinformatics to study the complete mode of HBV integration in hepatocellular carcinoma (HCC) cells. The results demonstrated that: 1) The HBV insertion sequences of HBV integration events accounted for 49.5% of the total HBV sequences. 2) Short insertion segments with the length of 0-1 kbp accounted for 50% and the long insertion segments (>3 kbp) accounted for 25% of HBV insertion events. 3)There were different HBV insertion length in the breakpoints formed within different regions. 4) The occurrence of HBV integration events was accompanied by more frequent structural variations. 5)Furthermore, multiple HBV integration patterns were confirmed based on complete HBV insertion sequences. Our research not only clarified a variety of perfect HBV integration models but also determined multiple specific features of HBV integration.
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