神经保护
细胞生物学
生物
神经退行性变
基因敲除
热休克蛋白
下调和上调
心理压抑
效应器
神经科学
基因表达
生物化学
细胞凋亡
基因
医学
疾病
病理
作者
Amandine Bastide,Diego Peretti,John R. P. Knight,Stefano Grosso,Ruth V. Spriggs,Xavier Pichon,Thomas Sbarrato,Anne Roobol,Jo Roobol,Davide Vito,Martin Bushell,Tobias von der Haar,C. Mark Smales,Giovanna R. Mallucci,Anne E. Willis
标识
DOI:10.1016/j.cub.2017.01.047
摘要
Cooling and hypothermia are profoundly neuroprotective, mediated, at least in part, by the cold shock protein, RBM3. However, the neuroprotective effector proteins induced by RBM3 and the mechanisms by which mRNAs encoding cold shock proteins escape cooling-induced translational repression are unknown. Here, we show that cooling induces reprogramming of the translatome, including the upregulation of a new cold shock protein, RTN3, a reticulon protein implicated in synapse formation. We report that this has two mechanistic components. Thus, RTN3 both evades cooling-induced translational elongation repression and is also bound by RBM3, which drives the increased expression of RTN3. In mice, knockdown of RTN3 expression eliminated cooling-induced neuroprotection. However, lentivirally mediated RTN3 overexpression prevented synaptic loss and cognitive deficits in a mouse model of neurodegeneration, downstream and independently of RBM3. We conclude that RTN3 expression is a mediator of RBM3-induced neuroprotection, controlled by novel mechanisms of escape from translational inhibition on cooling.
科研通智能强力驱动
Strongly Powered by AbleSci AI