Effects of Chinese yam ( Dioscorea oppositifolia L .) dietary supplementation on intestinal microflora, digestive enzyme activity and immunity in rainbow trout ( Oncorhynchus mykiss )

Aquaculture ResearchVolume 51, Issue 11 p. 4698-4712 ORIGINAL ARTICLE Effects of Chinese yam (Dioscorea oppositifolia L.) dietary supplementation on intestinal microflora, digestive enzyme activity and immunity in rainbow trout (Oncorhynchus mykiss) Fan Wang, Corresponding Author Fan Wang [email protected] orcid.org/0000-0001-8839-9535 School of Biological Science, Luoyang Normal University, Luoyang, China Correspondence Fan Wang, School of Biological Science, Luoyang Normal University, Luoyang 471022, China. Email: [email protected]Search for more papers by this authorHaifang Liu, Haifang Liu School of Energy and Environment Engineering, Zhongyuan University of Technology, Zhengzhou, ChinaSearch for more papers by this authorFei Liu, Fei Liu School of Biological Science, Luoyang Normal University, Luoyang, ChinaSearch for more papers by this authorWanguang Chen, Wanguang Chen School of Biological Science, Luoyang Normal University, Luoyang, ChinaSearch for more papers by this author Fan Wang, Corresponding Author Fan Wang [email protected] orcid.org/0000-0001-8839-9535 School of Biological Science, Luoyang Normal University, Luoyang, China Correspondence Fan Wang, School of Biological Science, Luoyang Normal University, Luoyang 471022, China. Email: [email protected]Search for more papers by this authorHaifang Liu, Haifang Liu School of Energy and Environment Engineering, Zhongyuan University of Technology, Zhengzhou, ChinaSearch for more papers by this authorFei Liu, Fei Liu School of Biological Science, Luoyang Normal University, Luoyang, ChinaSearch for more papers by this authorWanguang Chen, Wanguang Chen School of Biological Science, Luoyang Normal University, Luoyang, ChinaSearch for more papers by this author First published: 13 August 2020 https://doi.org/10.1111/are.14815Citations: 2Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Chinese yam (Dioscorea oppositifolia L.) extract is a product which concentrates many active ingredients. In this study, the effects of Chinese yam extract on the intestinal microbiota, digestive enzymes, and the immune response of rainbow trout (Oncorhynchus mykiss) were investigated. Fish were fed a basic diet (Con) and 0.1% (O1), 0.2% (O2) and 0.4% (O3) yam extract diet, respectively, for 8 weeks. After the feeding experiment, Actinobacteria, Firmicutes and Patescibacteria abundances at the phylum level in intestinal were significantly differential under the effect of yam respectively. Actinobacteria and Firmicutes phylum were significantly decreased in O1 group compared with control, while Patescibacteria was significantly increased in O3 group. Anaeroplasma, Candidatus_Saccharimonas, Ruminococcaceae_ UCG-014, Lactobacillus, Rikenella, Lachnospiraceae_NK4A136_group, [Eubacterium]_ coprostanoligenes_group, Bifidobacterium, Marvinbryantia and Uncultured abundances at the genus level were significantly differential under the effect of yam respectively. Bifidobacterium, Marvinbryantia and Candidatus_ Saccharimonas were significantly enriched in O3 group. KEGG and COG functional prediction on intestinal microflora revealed that yam has beneficial effects on metabolism, antibacterial and defence function. Serum immune factors and intestinal immune genes were significantly regulated in yam groups. In addition, yam also increased gastrointestinal digestive enzyme activities of rainbow trout. The results showed that yam could enhance gastrointestinal function, stimulate immunomodulatory effects, and induce intestinal microbiota changes, rendering beneficial effects to rainbow trout. Open Research DATA AVAILABILITY STATEMENT Data will be available upon request. Citing Literature Supporting Information Filename Description are14815-sup-0001-AppendixS1.docxWord document, 228.7 KB AppendixS1 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume51, Issue11November 2020Pages 4698-4712 RelatedInformation