Effects of Polysaccharide L01 from Laminaria on the Expression and Secretion of PAI- 1 in Cultured Human Umbilical Vein Endothelial Cell

Obejective To study the effects of polysaccharide L01 from Laminaria on the expression and secretion of plasminogen activator inhibitor- 1 (PAI- 1) stimulated by adrenaline in cultured human umbilical vein endothelial cells (HUVEC). Methods With adrenaline added, human umbilical vein endothelial cell line ECV- 304 was exposed to different concentrations of polysaccharide L01 of Laminaria and cultured for 24, 48, and 72 hours respectively in vitro. The levels of PAI- 1 in cultured supernants were measured with enzyme- linked immunosorbent assay( ELISA) .Reverse transcriptase chain reaction( RT- PCR) was used to detect the expression of PAI- 1 messenger RNA( mRNA) in HUVEC, and the related products were examined by density scan and half- quantitative analysis after electrophoresis. Results Stimulated by 10 μ g/mL adrenaline for 48h and 72 h, the levels of PAI- 1 in cultured supernants increased significantly (P 0.01, P 0.05), and the expression of PAI- 1 mRNA in the HUVEC was up- regulated. But the above effects were reduced by different concentration of polysaccharide L01 after cultured for 48 h and 72 h ( P 0.05 ). Conclusion Polysaccharide L01 can reduce the adrenaline- stimulated secretion and expression of PAI- 1 in cultured HUVEC. It is indicated that Polysaccharide L01 has the protective effect on vascular endothelial cells.