Proliferating and migrating effects of regenerating sea anemone Aulactinia stella cells- derived exosomes on human skin fibroblasts

Abstract This study evaluated the possible regenerative effects of proliferating sea anemone cells-derived exosomes on human foreskin fibroblasts (HFF). Water-based extracts from sea anemone Aulactinia stella tissue 48 h after regeneration was collected. The sea anemone, species were wounded from the middle of the column and the exosomes were extracted from 0 h, 24 h, 48 h, 72 h, and 96 h after wound induction. The extract and exosomes were separately analyzed on HFF using MTT for proliferation and in vitro wound healing for cell migration test. Additionally, an in-silico analysis was performed to investigate the protein-protein docking of regenerative Cnidarian exosomes protein contents with proliferation and migrations receptors present in HFF. The MTT showed extract or exosomes of sea anemone after 48 h of regeneration process had proliferation effects on HFF cells. At the cell migration test, both the extract and exosome had significant migratory effects on HFF cells after wound induction. Furthermore, our in-silico analysis identified potential binding affinities between the protein content of regenerative exosomes and receptors involved in HFF. Taken together, an optimized concentration of exosomes isolated from sea anemone A. stella could affect HFF regeneration and migration and accelerate wound healing in vitro.