纳米团簇
化学
活性氧
过氧化物酶
细胞毒性
氧化应激
谷胱甘肽
荧光
谷胱甘肽过氧化物酶
配体(生物化学)
细胞内
酶
生物物理学
组合化学
生物化学
体外
有机化学
受体
生物
物理
量子力学
作者
Mingxia Jiang,Xiaoyan Xu,S.Y. Liu,Liu Liu,Xuemei Wang,Hui Jiang
标识
DOI:10.1016/j.inoche.2024.112115
摘要
Nanozymes, as an alternative to natural enzymes, have received wide interests in recent years. Here, we have separated two main glutathione protected gold nanoclusters (GSH-Au NCs), namely, high fluorescent species with low peroxidase-like activity, and low fluorescent species with high peroxidase-like activity, from NCs prepared using different ratios of precursors. Additionally, the peroxidase-like activity of the latter species can be regulated by surface modification of a second thiolate ligand. As a representative, L-cysteine (L-Cys) modified GSH-Au NCs may exhibit both enhanced peroxidase-like activity (up to 5-fold) and improved fluorescent properties (∼3.3-fold) compared with GSH-Au NCs. The cytotoxicity and reactive oxygen species (ROS) level detection demonstrate that L-Cys modified NCs may cause effective cytotoxicity on breast cancer cells through the selective ROS generation. This study further expands the peroxidase-like kinetics of surface modified Au NCs and provides a new possibility for regulating the level of oxidative stress in cells.
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