谷胱甘肽
乳酸乳球菌
益生菌
活力测定
食品科学
抗氧化剂
化学
微生物学
细菌
乳酸
生物
生物化学
细胞
遗传学
酶
作者
Trung Hau Nguyen,Jin-Seong Kim,Hyuk-Ju Kwon,Chang-Ho Kang
出处
期刊:Fermentation
[Multidisciplinary Digital Publishing Institute]
日期:2023-02-17
卷期号:9 (2): 187-187
被引量:10
标识
DOI:10.3390/fermentation9020187
摘要
Lactic acid bacteria (LAB) and probiotics promise specific health benefits to their host. However, good storage stability is a prerequisite for their functioning and industrial use. This study aimed to evaluate glutathione (GSH) as a potential protective agent for improving microbial stability deteriorated by freeze-drying, freeze-thawing, and cold treatments. In this study, the optimal concentration of glutathione (50% w/w) was 1%, showing effective protection on the viability and stability of various LAB strains (Lactiplantibacillus plantarum MG4229 and MG4296, Lactococcus lactis MG5125, Limosilactobacillus fermentum MG4295, Lacticaseibacillus paracasei MG5012, and Bifidobacterium animalis ssp. lactis MG741). Glutathione-containing protectants considerably improved the viability of all of these strains after freeze-drying compared with non-coated probiotics. Survivability in the gastrointestinal (GI) tract, accelerated stability tests, and adhesion assays on intestinal epithelial cells were performed to determine whether glutathione enhances bacterial stability. Based on morphological observations, protectants containing GSH were coated onto the cell surface, resulting in effective protection against multiple external stress stimuli. The applicability of GSH as a new and effective protective agent can improve the stability and viability of various probiotics with anti-freezing and anti-thawing effects.
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