PTEN公司
张力素
生物
细胞周期
蛋白激酶B
细胞生长
下调和上调
PI3K/AKT/mTOR通路
胶质瘤
细胞生物学
癌症研究
信号转导
细胞周期蛋白D1
细胞
生物化学
基因
作者
Yawen Pan,Ruiqin Han,Qiao Li,Kang Wei,Qiang Dong,Hang Yin,Liang Niu,Junqiang Dai,Yunji Yan,Yuanping Su,Xuan Yao,He Zhang,Guoqiang Yuan,Yawen Pan
摘要
The cell cycle, a pivotal regulator of cell proliferation, can be significantly influenced by the phosphatase and tensin homolog (PTEN)/AKT signaling pathway's modulation of cyclin-related proteins. In our study, we discovered the crucial role of EEF1E1 in this process, as it appears to downregulate PTEN expression. Furthermore, our findings affirmed that EEF1E1 modulates downstream cell cycle-related proteins by suppressing the PTEN/AKT pathway. Cell cycle assay results revealed that EEF1E1 downregulation stunted the advancement of glioma cells in both the G1 and S phases. A suite of assays-Cell Counting Kit-8, colony formation, and ethyl-2'-deoxyuridine-substantiated that the EEF1E1 downregulation markedly curtailed glioma proliferation. We further validated this phenomenon through animal studies and coculture experiments on brain slices. Our comprehensive investigation indicates that EEF1E1 knockdown can effectively inhibit the glioma cell proliferation by regulating the cell cycle via the PTEN/AKT signaling pathway. Consequently, EEF1E1 emerges as a potential therapeutic target for glioma treatment, signifying critical clinical implications.
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