生物
妊娠期
怀孕
粪便
家养绵羊繁殖
谷氨酰胺
生理学
微生物群
早孕因素
动物科学
微生物学
生物化学
生物信息学
遗传学
氨基酸
作者
Zhiwu Wu,Yanyan Yang,Biao Wang,Kefyalew Gebeyew,Shaoxun Tang,Xuefeng Han,Zhixiong He,Zhiliang Tan
出处
期刊:Biology
[MDPI AG]
日期:2023-11-16
卷期号:12 (11): 1436-1436
标识
DOI:10.3390/biology12111436
摘要
Ewes undergo complex metabolic changes during pregnancy. Understanding the specific process of these changes is a necessary prerequisite in ewes for regulating and intervening in order to maintain pregnancies. However, there have been relatively few studies on the specific changes that occur in nutritional metabolism in pregnant ewes during early gestation, especially for some landrace ewes in highly cold areas. Therefore, this study aimed to (1) elucidate the changes in metabolites and microbial communities in pregnant ewes during early gestation using metabolomics and 16S ribosomal RNA gene (rDNA) amplicon sequencing approaches, and to (2) discover novel early pregnancy-induced biomarkers in the blood and faeces. Rams were placed together with ewes on D0 and removed on D45. During early gestation, blood and faecal samples were collected from ewes in a highly cold area for analysing the metabolites and microbial communities; these were retrospectively classified as the early gestation pregnant (EP) ewe group or the nonpregnant (NP) ewe group based on the lambing status recorded during the expected delivery period. The differences in the plasma biochemical parameters, plasma metabolites, and faecal microbial communities of pregnant and nonpregnant ewes were characterised. The GC, IL-6, O-acetyl-l-serine, L-glutamine, and 6-acetamido-2-oxohexanoic acid were screened out as potential biomarkers for evaluating the occurrence of early pregnancy. These novel early pregnancy-induced metabolites discovered in ewes might allow for the development of technologies to detect early pregnancies in sheep in highly cold areas.
科研通智能强力驱动
Strongly Powered by AbleSci AI