Chronic graft versus host disease detected by tissue-specific cell-free DNA methylation biomarkers

BACKGROUND. Accurate detection of graft versus host disease (GVHD) is a major challenge in the management of patients that undergo hematopoietic stem cell transplantation (HCT). Here we demonstrate the use of circulating cell-free DNA (cfDNA) for detection of tissue turnover and chronic GVHD (cGVHD) in specific organs.