Simultaneous imaging of Cysteine and viscosity in living cells with sharp contrast fluorescence between red and green by a dual-responsive fluorescence probe

Asmart dual-responsive fluorescence probe was designed and synthesized for simultaneous sensing of Cys and viscosity. The desired probe revealed high potency of imaging intracellular Cys and viscosity with selectivity and sensitivity, featured by the sharp contrast fluorescence between red and green. More importantly, the probe was successfully applied to simultaneously monitor the fluctuation of intracellular Cys and cytoplasmic viscosity in monensin- and LPS-induced cells.