沙蝇
生物
利什曼病
利什曼原虫
载体(分子生物学)
卢佐姆尼亚
聚合酶链反应
病毒学
查加西利什曼原虫
内脏利什曼病
精神科
皮肤利什曼病
亚属
静脉瘤
传输(电信)
动物
基因
遗传学
寄生虫寄主
分类学(生物学)
工程类
万维网
电气工程
计算机科学
重组DNA
作者
Byanca Regina de Paiva,Nágila Francinete Costa Secundino,João Cezar do Nascimento,Paulo Filemon Paolucci Pimenta,Eunice Aparecida Bianchi Galati,Hosana Aguiar Freitas de Andrade,Rosely S. Malafronte
出处
期刊:Acta Tropica
[Elsevier]
日期:2006-10-01
卷期号:99 (2-3): 252-259
被引量:75
标识
DOI:10.1016/j.actatropica.2006.08.009
摘要
Leishmaniasis is one of the most diverse and complex of all vector-borne diseases. Because it involves several overlapping species and sandfly vectors, the disease has a complex ecology and epidemiology. Adequate therapy and follow-up depend on parasitological diagnosis, but classical methods present several constraints when identifying species. We describe a polymerase chain reaction (PCR) which uses primers designed from mini-exon repetitive sequences that are specific for subgenus LeishmaniaViannia (PV), as well as sequences with specificity for genus (PG) that can distinguish between Leishmania species from other insect flagellates with minor differences in PCR products. For standardization, these PCR were tested in experimentally infected sandflies, and Leishmania infection in these insects was successfully confirmed. This methodology identified a 3.9% infection rate in field-captured phlebotomine sandflies from an endemic region in Brazil. Natural infection by Leishmania species was identified in three samples of Lutzomyia longipalpis, of which two were Leishmania (L.) chagasi and one Leishmania (L.) amazonensis. Irrespective of specific epidemiological conclusions, the method used in this study was able to identify Leishmania infections both in experimentally infected and field-captured phlebotomine sandflies, and could be a useful tool in epidemiological studies and strategic planning for the control of human leishmaniasis.
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