DC标志
静脉病毒
生物
内化
病毒学
内吞作用
病毒
树突状细胞
内体
细胞生物学
布尼亚病毒科
符号(数学)
甘露糖受体
受体
免疫学
抗原
遗传学
体外
数学分析
巨噬细胞
数学
作者
Pierre‐Yves Lozach,Andreas Kühbacher,Roger Meier,R. C. Mancini,David Bitto,Michèle Bouloy,Ari Helenius
标识
DOI:10.1016/j.chom.2011.06.007
摘要
During natural transmission, bunyaviruses are introduced into the skin through arthropod bites, and dermal dendritic cells (DCs) are the first to encounter incoming viruses. DC-SIGN is a C-type lectin highly expressed on the surface of dermal DCs. We found that several arthropod-borne phleboviruses (Bunyaviridae), including Rift Valley fever and Uukuniemi viruses, exploit DC-SIGN to infect DCs and other DC-SIGN-expressing cells. DC-SIGN binds the virus directly via interactions with high-mannose N-glycans on the viral glycoproteins and is required for virus internalization and infection. In live cells, virus-induced clustering of cell surface DC-SIGN could be visualized. An endocytosis-defective mutant of DC-SIGN was unable to mediate virus uptake, indicating that DC-SIGN is an authentic receptor required for both attachment and endocytosis. After internalization, viruses separated from DC-SIGN and underwent trafficking to late endosomes. Our study provides real-time visualization of virus-receptor interactions on the cell surface and establishes DC-SIGN as a phlebovirus entry receptor.
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