自愈水凝胶
明胶
细胞外基质
组织工程
细胞包封
间充质干细胞
干细胞
脂肪组织
活力测定
材料科学
化学
细胞分化
细胞
细胞生物学
生物医学工程
高分子化学
生物化学
生物
基因
医学
作者
Bapi Sarker,Tobias Zehnder,Subha Narayan Rath,Raymund E. Horch,Ulrich Kneser,Rainer Detsch,Aldo R. Boccaccını
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2017-07-07
卷期号:3 (8): 1730-1737
被引量:63
标识
DOI:10.1021/acsbiomaterials.7b00188
摘要
Alginate-based hydrogels are extensively used matrices for cell encapsulation, but they need to be modified to recapitulate chemical, microstructural, and mechanical properties of the native extracellular matrix. Like other cell types, mesenchymal stem cells exhibit rounded and clustered morphologies when they are embedded in alginate hydrogels. In this study, we use covalently cross-linked oxidized alginate-gelatin hydrogels to encapsulate human adipose-derived stem cells in order to investigate cell growth, viability, and morphology during osteogenic differentiation taking advantage of the different physicochemical properties of this modified alginate-based hydrogel in comparison to those of the pristine alginate hydrogel. We investigate the effect of hydrogel compositions on stem cell behavior in 3D. Higher viability and the spreading morphology of encapsulated cells with interconnected networks were observed in high gelatin containing compositions. More filopodial protrusions from multicellular nodules were noticed during osteogenic differentiation in the hydrogels having a high amount of gelatin, confirming their suitability for cell encapsulation and bone tissue engineering applications.
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