Immobilization and kinetic study of lipase enzymes from Lactobacillus plantarum and Lactobacillus brevis

Enzymes are biological catalysts which are used in promoting the rate of reactions. The repeated use of enzyme in different solvents results in loss of enzyme activity and cannot be economically recovered. It is important to choose an enzyme which has fastest reaction rate, efficiency and stability for industrial scale production. Hence immobilization and enzyme kinetics play an important role in enzymology. In the present study lipase from Lactobacillus brevis and Lactobacillus plantarum were immobilized in sodium alginate beads and kinetic study was done using pnitrophenyl palm itate as substrate. Upon immobilization the enzyme showed optimal conditions of pH7 and 40 C temperature for Lactobacillus brevis and pH 65 and 45 C temperature for Lactobacillus plantarum. In terms of stability and efficiency, the immobilization of lipase by sodium alginate beads showed good results over expressed enzyme and can be used in large scale production of industrial products.