“Click” on Alkynylated Carbon Quantum Dots: An Efficient Surface Functionalization for Specific Biosensing and Bioimaging

Abstract Surface functionalization is an essential pre requisite for wide and specific applications of nanoparticles such as photoluminescent (PL) carbon quantum dots (CQDs), but it remains a major challenge. In this report, alkynylated CQDs, prepared from carboxyl‐rich CQDs through amidation with propargylamine in the presence of 1,1′‐carbonyldiimidazole, were modified efficiently with azido molecular beacon DNA through a copper(I)‐catalyzed alkyne–azide cycloaddition reaction (CuAAC). As a proof‐of‐concept, the DNA‐modified CQDs are then bonded with gold nanoparticles (AuNPs, 5 nm) through a gold–sulfur bond. Owing to the emission enhancement, this complex can then be applied to the recognition of a single‐base‐ mismatched target. The same functionalizing strategy applied to click the alkynylated CQDs with a nuclear localization sequence (NLS) peptide showed that the NLS‐modified CQDs could target the nuclei specifically. These results indicate that surface functionalization of CQDs through a nonstoichiometric copper chalcogenide nanocrystal‐ (nsCuCNC‐) catalyzed click reaction is efficient, and has significant potential in the fields of biosensing and bioimaging.