胰岛素
孵化
内科学
内分泌学
格列本脲
小岛
糖尿病
下调和上调
化学
生物
医学
基因
生物化学
作者
Eiji Yamato,Hiroshi Ikegami,Yasuhiro Tahara,Minoru Fukuda,Tepyon Cha,Y. Kawaguchi,Yoshihiko Fujioka,Yukio Noma,Kenji Shima,Takeshi Ogihara
标识
DOI:10.1006/bbrc.1994.1232
摘要
Long-term glyburide therapy has been reported to improve glucose-induced insulin secretion in patients with NIDDM. We examined the effects of glyburide on the synthesis release of insulin and insulin gene expression in isolated islets in vitro. Incubation with glyburide (500 ng/ml) significantly increased insulin release without affecting the insulin content. The PPI mRNA level was not increased after incubation for 1 h but was increased after incubation for 20 h. Incubation with 5 ng/ml of glyburide for 1 h or 20 h had no effect on the content or release of insulin, but incubation with 5 ng/ml of glyburide for 20 h significantly increased the PPI mRNA level and enhanced insulin release induced by 11 mM glucose. These results suggest that a high concentration of glyburide stimulates insulin release directly, while a low concentration of glyburide increases the PPI mRNA level and may thereby enhance glucose-induced insulin release.
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