地氯酸
适体
金黄色葡萄球菌
核糖核酸
指数富集配体系统进化
微生物学
SELEX适体技术
葡萄球菌
生物
细菌
生物化学
分子生物学
基因
遗传学
作者
Seung Ryul Han,Seong-Wook Lee
标识
DOI:10.1007/s13213-013-0720-z
摘要
Staphylococcus aureus is a major foodborne pathogen. Gram-positive bacteria have unique teichoic acids as cell-wall components. In order to identify ligands specific to the bacteria, we developed an RNA aptamer against the teichoic acid of Staphylococcus aureus using SELEX technology. To this end, we used a polystyrene 96-well-based selection method and confirmed the binding activity of the RNA aptamer to the teichoic acid using real-time PCR. Of note, the teichoic acid-specific RNA aptamer was observed to bind to S. aureus bacterial cells also. This RNA aptamer could therefore be useful as a diagnostic ligand against S. aureus-associated foodborne illness.
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