Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems

A new thiobarbituric acid method for the estimation of the malonyl dialdehyde produced as a result of lipid peroxidation is described in which the trimethine colored substance is estimated in alkaline solution. Alkaline pyridine-butanol mixture dissolves both proteins and lipids that are precipitated or opalescent during the reaction, yielding a more accurate MDA estimation as well as simplifying the procedure.