Laccase as a New Enzymatic Label for Enzyme Immunoassay

A new immunochemical reagent is suggested containing as an enzyme marker laccase obtained from the cultural liquid of basidial fungi Coriolus hirsutus. The feasibility of immuno-laccase conjugates in different versions of immunoanalysis (sandwich, competitive and indirect enzyme immunoassay) was demonstrated. The assay based on antibodvlaccase conjugates is simpler than that employing antibody-peroxidase conjugates, since in the former case air oxygen is used as the second substrate of the enzymatic reaction.