酵母
双杂交筛选
酿酒酵母
鉴定(生物学)
化学
生物
生物化学
计算生物学
蛋白质-蛋白质相互作用
真菌蛋白
基因
作者
Hazel McLellan,Miles R. Armstrong,Paul R. J. Birch
出处
期刊:Methods of Molecular Biology
日期:2021-01-01
卷期号:2354: 95-110
标识
DOI:10.1007/978-1-0716-1609-3_4
摘要
Yeast two-hybrid (Y2H) is a technique used to identify protein-protein interactions. It relies on interacting proteins bringing the two domains of a split transcription factor into close proximity, thereby reconstituting its ability to activate reporter genes. There are many variations on this technique. Here we provide an adapted protocol based on the Invitrogen ProQuest™ Two-Hybrid system, which has been used successfully to screen over 60 Phytophthora infestans pathogen effector proteins to identify candidate-interacting proteins in Solanum tuberosum, and has been used to identify many potato-potato protein-protein interactions.
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