膜
膜曲率
生物物理学
细胞器生物发生
细胞膜弹性
小泡
细胞膜
膜蛋白
细胞生物学
化学
整体膜蛋白
外周膜蛋白
生物
生物发生
生物化学
基因
作者
Yuan Feng,Haleh Alimohamadi,Brandon Bakka,Andrea N. Trementozzi,Kasey J. Day,Nicolas L. Fawzi,Padmini Rangamani,Jeanne C. Stachowiak
标识
DOI:10.1073/pnas.2017435118
摘要
Membrane bending is a ubiquitous cellular process that is required for membrane traffic, cell motility, organelle biogenesis, and cell division. Proteins that bind to membranes using specific structural features, such as wedge-like amphipathic helices and crescent-shaped scaffolds, are thought to be the primary drivers of membrane bending. However, many membrane-binding proteins have substantial regions of intrinsic disorder which lack a stable three-dimensional structure. Interestingly, many of these disordered domains have recently been found to form networks stabilized by weak, multivalent contacts, leading to assembly of protein liquid phases on membrane surfaces. Here we ask how membrane-associated protein liquids impact membrane curvature. We find that protein phase separation on the surfaces of synthetic and cell-derived membrane vesicles creates a substantial compressive stress in the plane of the membrane. This stress drives the membrane to bend inward, creating protein-lined membrane tubules. A simple mechanical model of this process accurately predicts the experimentally measured relationship between the rigidity of the membrane and the diameter of the membrane tubules. Discovery of this mechanism, which may be relevant to a broad range of cellular protrusions, illustrates that membrane remodeling is not exclusive to structured scaffolds but can also be driven by the rapidly emerging class of liquid-like protein networks that assemble at membranes.
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