Preparation and disinfection properties of graphene oxide/trichloroisocyanuric acid disinfectant

Due to the impact of the new crown epidemic in recent years, disinfectants have played an increasingly important role, so the research and development of new high-efficiency nano-disinfectants are urgent issues. In this study, graphene oxide (GO) was first prepared by the modified Hummer method. Then, the GO/trichloroisocyanuric acid (TCCA) composite was prepared by loading TCCA into GO with the blending method. X-ray diffraction, scanning electron microscopy, transmission electron microscopy, x-ray photoelectron spectroscopy and atomic force microscopy were used to characterize the composite. The results showed that TCCA was successfully loaded on the surface of GO or intercalated among GO layers. Next, the antibacterial performance of the composite againstEscherichia coliandStaphylococcus aureuswas tested by the 96-well plate assay. A bactericidal kinetic curve, bacterial inhibition tests, and the mechanism of bacterial inhibition were discussed. The results showed that the minimum inhibitory concentration (MIC) of the GO/TCCA composite (GO:TCCA ratio = 1:50) was 327.5μg ml-1againstE. coliand 655μg ml-1againstS. aureus. At the MIC, the inhibition rate of the GO/TCCA composite exceeded 99.46% againstE. coliand 99.17% againstS. aureus. The bactericidal kinetic curves indicate that the GO/TCCA composite has an excellent bactericidal effect againstE. coliandS. aureus.