异源双工
T细胞受体
淋巴增殖性病變
免疫球蛋白重链
分子生物学
聚合酶链反应
基因重排
生物
抗体
基因
免疫球蛋白轻链
淋巴瘤
免疫学
T细胞
遗传学
免疫系统
作者
Elke Boone,Kim C. Heezen,Patricia J. T. A. Groenen,Anton W. Langerak
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 77-103
被引量:6
标识
DOI:10.1007/978-1-4939-9151-8_4
摘要
Assessment of the presence of clonal lymphoproliferations via polymerase chain reaction (PCR)-based analysis of rearranged immunoglobulin (IG) or T-cell receptor (TR) genes is a valuable method in the diagnosis of suspect lymphoproliferative disorders. Additionally, this methodology can be used for evaluating dissemination of lymphoma cells and for studying the clonal relationship between multiple (different locations) or consecutive (over time) lymphomas. Here we describe an integrated approach to assess clonality via analysis of Ig heavy chain (IGH), Ig kappa (IGK), TCR beta (TRB), and TCR gamma (TRG) gene rearrangements, based on the standardized multiplex PCRs as originally developed by the European BIOMED-2 consortium. The described protocol covers the pre-analytical phase of DNA isolation (from formalin-fixed paraffin-embedded and fresh tissues, body fluids, peripheral blood, and bone marrow), the analytical phase of PCR GeneScan and heteroduplex analysis, and the post-analytical interpretation of the obtained profiles, following established guidelines.
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