周质间隙
单元格信封
生物
细胞生物学
细胞质
肽聚糖
细菌外膜
内膜
衰减器(电子)
调节器
基因
遗传学
大肠杆菌
光学
衰减
物理
线粒体
作者
Nahla A. Hussein,Seung-Hyun Cho,Géraldine Laloux,Rania Siam,Jean-François Collet
出处
期刊:PLOS Genetics
日期:2018-05-31
卷期号:14 (5): e1007398-e1007398
被引量:39
标识
DOI:10.1371/journal.pgen.1007398
摘要
In enterobacteria, the Rcs system (Regulator of capsule synthesis) monitors envelope integrity and induces a stress response when damages occur in the outer membrane or in the peptidoglycan layer. Built around a two-component system, Rcs controls gene expression via a cascade of phosphoryl transfer reactions. Being particularly complex, Rcs also involves the outer membrane lipoprotein RcsF and the inner membrane essential protein IgaA (Intracellular growth attenuator). RcsF and IgaA, which are located upstream of the phosphorelay, are required for normal Rcs functioning. Here, we establish the stress-dependent formation of a complex between RcsF and the periplasmic domain of IgaA as the molecular signal triggering Rcs. Moreover, molecular dissection of IgaA reveals that its negative regulatory role on Rcs is mostly carried by its first N-terminal cytoplasmic domain. Altogether, our results support a model in which IgaA regulates Rcs activation by playing a direct role in the transfer of signals from the cell envelope to the cytoplasm. This remarkable feature further distinguishes Rcs from other envelope stress response systems.
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