化学
荧光假单胞菌
金黄色葡萄球菌
抗菌活性
细菌
微生物学
细菌细胞结构
生物化学
生物
遗传学
作者
Shimo Kang,Fanhua Kong,Xinyang Shi,Hongjiao Han,Boyuan Guan,Mei Yang,Xiaohong Cao,Dongbing Tao,Yan Zheng
出处
期刊:Food Control
[Elsevier]
日期:2020-02-01
卷期号:108: 106876-106876
被引量:77
标识
DOI:10.1016/j.foodcont.2019.106876
摘要
Lactobionic acid (LBA), a polyhydroxy organic acid, is supposed to be a promising candidate for use as multifunctional food additive due to its ability of antibacterial, anti-obesity, etc. The antibacterial activity of LBA has rarely been reported, thus the antibacterial activity and mechanism of LBA against Pseudomonas fluorescens and methicillin-resistant Staphylococcus aureus were investigated. The results of antibacterial activity showed that P. fluorescens was more susceptible to LBA with the lowest minimum inhibitor concentration/minimum bactericidal concentration and that LBA could completely inactivate the selected bacteria. The alkaline phosphatase activity assay, flow cytometry, protein leakage, and sodium dodecyl sulfate polyacrylamide gel electrophoresis demonstrated that LBA could disrupt the integrity of the cell wall and membrane, and the content and activity of bacterial proteins. Scanning electron microscopy and transmission electron microscopy results showed obvious morphological and ultrastructural changes in the bacterial cells, further confirming the cell wall and membrane damages caused by LBA. Moreover, LBA could interact with bacterial DNA via intercalation, to disturb the normal cellular functions. LBA also exhibited antibiofilm formation activity and the ability to inhibit bacterial growth in whole milk. Overall, LBA is expected to be an alternative for novel multifunctional food additive with bacteriostatic action in food industries.
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