微尺度热泳
热泳
微尺度化学
化学
运输机
ATP结合盒运输机
生物化学
纳米技术
材料科学
心理学
纳米流体
数学教育
纳米颗粒
基因
作者
Tanja Bartoschik,Amit Gupta,Beate Kern,Andrew Hitchcock,Nathan B. P. Adams,Nuska Tschammer
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 51-62
被引量:5
标识
DOI:10.1007/978-1-0716-0724-4_2
摘要
The combination of MicroScale Thermophoresis (MST) and near-native site-specific His-tag labeling enables simple, robust, and reliable determination of the binding affinity between proteins and ligands. To demonstrate its applicability for periplasmic proteins, we provide a detailed protocol for determination of the binding affinity of phosphite to three ABC transporter periplasmic-binding proteins from environmental microorganisms. ABC transporters are central to many important biomedical phenomena, including resistance of cancers and pathogenic microbes to drugs. The protocol described here can be used to quantify protein-ligand and protein-protein interactions for other soluble, membrane-associated and integral membrane proteins.
科研通智能强力驱动
Strongly Powered by AbleSci AI