酶
化学
配体(生物化学)
生物化学
底物特异性
劈理(地质)
基质(水族馆)
结晶
组合化学
立体化学
生物
有机化学
受体
生态学
断裂(地质)
古生物学
作者
Leonie G Graf,Emil A. P. Michels,Yelwin Yew,Weidong Liu,Gottfried J. Palm,Gert Weber
出处
期刊:Methods in Enzymology
日期:2021-01-01
卷期号:: 337-356
被引量:4
标识
DOI:10.1016/bs.mie.2020.12.015
摘要
The concept of biocatalytic PET degradation for industrial recycling processes had made a big step when the bacterium Ideonella sakaiensis was discovered to break PET down to its building blocks at ambient temperature. This process involves two enzymes: cleavage of ester bonds in PET by PETase and in MHET, the resulting intermediate, by MHETase. To understand and further improve this unique capability, structural analysis of the involved enzymes was aimed at from early on. We describe a repertoire of methods to this end, including protein expression and purification, crystallization of apo and substrate-bound enzymes, and modeling of PETase complexed with a ligand.
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