里氏木霉
拉伤
纤维素酶
生物
突变
野生型
突变体
木霉菌
重组DNA
微生物学
生物技术
酶
食品科学
生物化学
基因
解剖
作者
Robyn Peterson,Helena Nevalainen
出处
期刊:Microbiology
[Microbiology Society]
日期:2012-01-01
卷期号:158 (1): 58-68
被引量:404
标识
DOI:10.1099/mic.0.054031-0
摘要
The hypersecreting mutant Trichoderma reesei RUT-C30 (ATCC 56765) is one of the most widely used strains of filamentous fungi for the production of cellulolytic enzymes and recombinant proteins, and for academic research. The strain was obtained after three rounds of random mutagenesis of the wild-type QM6a in a screening program focused on high cellulase production and catabolite derepression. Whereas RUT-C30 achieves outstanding levels of protein secretion and high cellulolytic activity in comparison to the wild-type QM6a, recombinant protein production has been less successful. Here, we bring together and discuss the results from biochemical-, microscopic-, genomic-, transcriptomic-, glycomic- and proteomic-based research on the RUT-C30 strain published over the last 30 years.
科研通智能强力驱动
Strongly Powered by AbleSci AI