7strains degrading oil were isolated from long-term oil-contaminated soil and they were numbered as bacteria 2-1,bacteria 7-1,bacteria 1-2,bacteria5-2,bacteria 7-2,bacteria oil 3,bacteria oil 5,of which bacteria 2-1and bacteria 7-1were identified as Serratia marcescens,bacteria 1-2as Raoultella planticola,bacteria 5-2,bacteria oil 3,and bacteria oil 5as Klebsiella genus,bacteria 7-2as Bacillus cereus by morphological observation,Biolog identification and 16SrDNA gene sequence analysis.Bacteria 1-2,bacteria 5-2and bacteria 7-2enabled the surface tension of the fermentation liquid reduce from 36.1mN/m to 20.2mN/m, 20.74mN/m and 21.78mN/m,indicating the surfactants produced by the bacteriums have a strong emulsification crude capacity and show great potential.