生物传感器
电容
阶段(地层学)
氧化三甲胺
材料科学
炸薯条
三甲胺
纳米技术
化学
电极
计算机科学
生物
生物化学
电信
物理化学
古生物学
作者
Wei‐Cheng Lin,Yi‐Ling Hsieh,P. Ho,Rihua Zeng,Chun‐Ting Hsieh,Ming‐Chiu Chang,Che‐Chung Yeh,Chi-Jen Lo,Mei‐Ling Cheng,Chien‐Hung Liao
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2024-02-13
卷期号:9 (2): 638-645
标识
DOI:10.1021/acssensors.3c01699
摘要
A demonstration of an off-chip capacitance array sensor with a limit of detection of 1 μM trimethylamine N-oxide (TMAO) to diagnose a chronic metabolism disease in urine is presented. The improved Cole-Cole model is employed to determine the parameters of R_catalyzed, C_catalyzed, and Rp_catalyzed, enabling the prediction of the catalytic resistance of enzyme, reduction effects of the analyte, and characterize the small signal alternating current properties of ionic strength caused by catalysis. Based on the standard solutions, we investigate the effects of pixel geometry parameters, driving electrode width, and sensing electrode width on the electrical field change of the off-chip capacitance sensor; the proposed off-chip sensor with readout system-on-chip exhibits a high sensitivity of 21 analog-to-digital converter counts/μM TMAO (or 2.5 mV/μM TMAO), response time of 1 s, repetition of 98.9%, and drift over time of 0.5 mV. The proposed off-chip sensor effectively discriminates TMAO in a phosphate-buffered saline solution based on minute changes in capacitance induced by the TorA enzyme, resulting in a discernible 2.15% distinction. These measurements have been successfully corroborated using the conventional cyclic voltammetry method, demonstrating a mere 0.024% variance. The off-chip sensor is crafted with a specific focus on detecting TMAO, achieved by excluding any reduction reactions between the TMAO-specific enzyme TorA and the compounds creatine and creatinine present in urine. This deliberate omission ensures that the sensor's attention remains solely on TMAO, thereby enhancing its precision in achieving accurate and reliable TMAO detection.
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