Multipoint MicroLED probes for selective neural stimulation in optogenetics

Abstract Advances in neuroscience necessitate precise methods for analyzing and controlling neural activity. Optogenetics, which integrates genetic engineering with optical stimulation, has emerged as a transformative approach. However, traditional methods relying on genetic specificity have limitations in spatial precision. To overcome this, we developed a multi-point micro-light-emitting LED (MicroLED) probe that enables independent control of spatially confined light delivery. Through light scattering simulations and in vivo optogenetic experiments targeting the hippocampal CA3-CA1 circuit in mice, we demonstrate that selective LED activation can precisely induce neural activity without unintended stimulation of adjacent regions. Our findings highlight the importance of optimizing optical intensity and spatial resolution to enhance optogenetic specificity, bridging the gap between engineering and neuroscience for improved neural circuit analysis.