Pterostilbene: A natural compound for anti‐ageing in sebaceous gland cells and fibroblasts

Abstract Objective The skin, as the largest organ of the body, plays a critical role in homeostasis and protecting against environmental stressors. Sebaceous glands (SGs) produce sebum, which forms a lipid barrier essential for preventing dehydration and defending against oxidative and microbial damage. However, ageing and environmental factors such as UV exposure and pollution impair SG function, leading to skin dryness and loss of radiance. Pterostilbene, a natural antioxidant with anti‐inflammatory effects, has not been extensively studied for its potential impact on ageing SG cells. This study aims to investigate the protective effects of pterostilbene on SG function under UV‐induced damage and its role in collagen repair in UV‐exposed fibroblasts. Methods To examine the effects of pterostilbene on UVA‐induced damage, an in vitro model of UV‐exposed sebocytes was developed. The influence of pterostilbene on lipid synthesis, reactive oxygen species (ROS) levels and gene expression related to senescence and inflammation was assessed. In parallel, the effects of pterostilbene on collagen synthesis (types I and III) and ROS levels in UV‐exposed fibroblasts were analysed. Super‐resolution fluorescence microscopy was utilized to visualize collagen production and F‐actin integrity in fibroblasts treated with pterostilbene. Results Pterostilbene increased lipid synthesis by 16% and reduced ROS levels by 62.43% in UV‐damaged sebocytes. Gene expression analysis revealed upregulation of antioxidant genes (Nrf2, Sirt6) and downregulation of differentiation‐related genes (Blimp1, c‐Myc). In UV‐exposed fibroblasts, pterostilbene significantly enhanced collagen types I and III levels and reduced ROS by 40.07%. High‐resolution microscopy showed that pterostilbene restored the distribution of F‐actin in fibroblasts affected by UVA. Conclusion This study demonstrates that pterostilbene effectively protects against UVA‐induced damage in both sebocytes and fibroblasts. By promoting lipid synthesis, reducing oxidative stress, and stimulating collagen production, pterostilbene presents a promising natural ingredient for anti‐ageing skincare formulations. Its multifunctional mechanisms suggest its potential to enhance skin health by supporting sebaceous gland function and improving overall skin resilience.