生物
发起人
维管形成层
基因
木质部
增强子
转基因
基因表达
格斯报告系统
形成层
报告基因
韧皮部
计算生物学
遗传学
植物
作者
Wei Zeng,Jianyang Xue,Yang Song,Shiqi Wang,Ningning Chen,Lichao Huang,Cheng Jiang,Mengzhu Lu,Jin Zhang
出处
期刊:Plant Science
[Elsevier]
日期:2024-07-01
卷期号:344: 112083-112083
标识
DOI:10.1016/j.plantsci.2024.112083
摘要
Due to the extended generation cycle of trees, the breeding process for forest trees tends to be time-consuming. Genetic engineering has emerged as a viable approach to expedite the genetic breeding of forest trees. However, current genetic engineering techniques employed in forest trees often utilize continuous expression promoters such as CaMV 35S, which may result in unintended consequences by introducing genes into non-target tissues. Therefore, it is imperative to develop specific promoters for forest trees to facilitate targeted and precise design and breeding. In this study, we utilized single-cell RNA-Seq data and co-expression network analysis during wood formation to identify three vascular tissue-specific genes in poplar, PP2-A10, PXY, and VNS07, which are expressed in the phloem, cambium/expanding xylem, and mature xylem, respectively. Subsequently, we cloned the promoters of these three genes from '84K' poplar and constructed them into a vector containing the eyGFPuv visual selection marker, along with the 35S mini enhancer to drive GUS gene expression. Transgenic poplars expressing the ProPagPP2-A10::GUS, ProPagPXY::GUS, and ProPagVNS07::GUS constructs were obtained. To further elucidate the tissue specificity of these promoters, we employed qPCR, histochemical staining, and GUS enzyme activity. Our findings not only establish a solid foundation for the future utilization of these promoters to precisely express of specific functional genes in stems but also provide a novel perspective for the modular breeding of forest trees.
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