Directed evolution of an alcohol dehydrogenase for the desymmetric reduction of 2,2-disubstituted cyclopenta-1,3-diones by enzymatic hydrogen transfer

Directed evolution of carbonyl reductase TbADH created mutant Tb2 with balanced activity toward ethyl secodione and isopropanol, enabling the desymmetric reduction of ethyl secodione to give (13 R ,17 S )-ethyl secol with 94% yield, >99% ee and >99% de.