Evaluation of in vitro antioxidant potential of Anisomeles indica Kuntze and exploration of its bioactive phytoconstituents

Introduction and Aim: Anisomeles indica Kuntze (A. indica) is employed to treat a wide range of illnesses. The Present study was aimed at establishing preliminary phytochemical screening, UV-Visible spectrophotometric, FT-IR analysis, and evaluation of biological activities of A. indica extracts. Materials and Methods: A. indica leaves were employed for the Soxhlet extraction. Furthermore, the crude extracts were utilized for phytochemical analysis and quantitative estimations of phenolics and flavonoids. UV-Vis spectrophotometric, and FT-IR analysis provided further evidence for the existence of bioactive constituents in A. indica extracts. The A. indica extracts were assessed for antioxidant potential by DPPH and metal chelation activity. Results: The findings illustrated that A. indica methanol extract was found to possess the highest yield. The preliminary phytochemical screening, UV-Vis spectrophotometric, and FT-IR fingerprint analysis provided evidence for the existence of significant bioactive constituents. A.indica methanol extract has significant total phenol, flavonoid content, and TAC (total antioxidant capacity) among all extracts. These characteristics are attributed to substantial antioxidant activity and metal-chelating activity. Conclusion: The findings of this study imply that A. indica extract possess antioxidant activity as evaluated by the potential DPPH radical scavenging and chelate metal ions. These characteristics are interconnected to the high flavonoid and phenol content, and distinctive secondary metabolites. The finding indicates that A.indica is abundant in active phytoconstituents, which also offer a vital source for effective therapeutic management.