András T. Deak,Claire Jean-Quartier,Alexander I. Bondarenko,Lukas N. Groschner,Roland Malli,Wolfgang F. Graier,Markus Waldeck-Weiermair
出处
期刊:Methods in molecular biology日期:2021-01-01卷期号:: 173-191
标识
DOI:10.1007/978-1-0716-1266-8_13
摘要
Mitochondrial Ca2+ uptake regulates mitochondrial function and contributes to cell signaling. Accordingly, quantifying mitochondrial Ca2+ signals and elaborating the mechanisms that accomplish mitochondrial Ca2+ uptake are essential to gain our understanding of cell biology. Here, we describe the benefits and drawbacks of various established old and new techniques to assess dynamic changes of mitochondrial Ca2+ concentration ([Ca2+]mito) in a wide range of applications.