壳聚糖
移植
氧化应激
化学
组织工程
脚手架
自愈水凝胶
活性氧
抗氧化剂
生物物理学
生物医学工程
高分子化学
生物化学
外科
医学
生物
作者
Yueping Guo,Youyang Qu,Jiaqi Yu,Lili Song,Simin Chen,Zhenmiao Qin,Jingwen Gong,Haihe Zhan,Yujie Gao,Junqing Zhang
标识
DOI:10.1016/j.ijbiomac.2022.01.030
摘要
Stem cell transplantation technology provides the cell reconstruction of damaged heart a completely new therapy approach. Due to the inappropriate microenvironment such as reactive oxygen radicals caused by ischemic infarct, the survival and retention rates of cell transplantation are not desirable. A thermo sensitive chitosan-vitamin C (CSVC) hydrogel scaffold was developed to reduce oxidative stress injury after myocardial infarction, thereby increasing the cell survival rate of cell transplantation. Vitamin C was conjugated on the chitosan chain by electrostatic adsorption. Compared to chitosan, CSVC complex had a higher solubility and stronger antioxidant property. CSVC hydrogel has suitable gelation time and injectable properties. Scanning electron microscopy showed that chitosan hydrogels had three-dimensional porous structure with irregular pores interconnected throughout the construct. Live/dead and H&E staining results showed that CSVC hydrogel can support the survival and adhesion of cardiomyocytes. Compared with chitosan hydrogel, CSVC hydrogel can clearly improve the survival of cardiomyocytes and reduce the ROS level under H2O2-induced oxidative stress conditions. These results suggest that CSVC hydrogel has the potential to support the survival of cardiomyocytes in tissue engineering.
科研通智能强力驱动
Strongly Powered by AbleSci AI