小胶质细胞
碲化镉光电
化学
代谢组学
生物物理学
材料科学
生物
纳米技术
炎症
免疫学
色谱法
作者
Keyu He,Xue Li,Tingting Wei,Na Liu,Yan Wang,Linyue Zou,Changcun Bai,Yao Ying,Tianshu Wu,Lu Kong,Ting Zhang,Xue Yang,Meng Tang
出处
期刊:Chemosphere
[Elsevier]
日期:2020-05-01
卷期号:246: 125629-125629
被引量:13
标识
DOI:10.1016/j.chemosphere.2019.125629
摘要
In this study, a metabolomic analysis was used to reveal the neurotoxicity of the CdTe/ZnS QDs via microglia polarization. A gas chromatography-mass spectrometer (GC-MS) was applied to uncover the metabonomic changes in microglia (BV-2 cell line) after exposure to 1.25 μM CdTe/ZnS QDs. 11 annotated metabolic pathways (KEGG database) were significantly changed in all exposed groups (3 h, 6 h, 12 h), 3 of them were related to glucose metabolism. The results of the Seahorse XFe96 Analyzer indicated that the CdTe/ZnS QDs increased the glycolysis level of microglia by 86% and inhibited the aerobic respiration level by 54% in a non-hypoxic environment. In vivo study, 3 h after the injection of CdTe/ZnS QDs (2.5 mM) through the tail vein in mice, the concentration of the CdTe/ZnS QDs in hippocampus reached the peak (1.25 μM). The polarization level of microglia (Iba-1 immunofluorescence) increased 2.7 times. In vitro study, the levels of the extracellular TNF-α, IL-1β and NO of BV-2 cells were all increased significantly after a 6 h or 12 h exposure. According to the results of the Cell Counting Kit-8, after a 6 h or 12 h exposure to the CdTe/ZnS QDs, the exposed microglia could significantly decrease the number of neurons (HT-22 cell line). This study proved that CdTe/ZnS QDs could polarize microglia in the brain and cause secondary inflammatory damage to neurons. There are potential risks in the application of the CdTe/ZnS QDs in brain tissue imaging.
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