淘洗
流式细胞术
分离(微生物学)
单核细胞
分泌物
细胞生物学
生物
化学
免疫学
分子生物学
生物化学
微生物学
有机化学
作者
Larry M. Wahl,Sharon M. Wahl,Lesley E. Smythies,Phillip D. Smith
标识
DOI:10.1002/0471142735.im0706as70
摘要
This unit describes the isolation of monocytes from lymphocytes by adherence, gradient sedimentation on colloidal silica particles, and flow cytometry. Because the first two methods can result in cell activation (induction of gene expression or protein secretion), and the third is technically difficult, a fourth protocol is presented which describes counterflow centrifugal elutriation. This latter procedure can be used to isolate large numbers of purified, nonactivated monocytes.
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