| 标题 |
 Immunophenotype characterization of rat mesenchymal stromal cells 大鼠间充质基质细胞的免疫表型特征
相关领域
间充质干细胞
CD90型
间质细胞
祖细胞
免疫分型
细胞生物学
人口
生物
骨髓
细胞疗法
免疫学
干细胞
癌症研究
流式细胞术
川地34
医学
环境卫生
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| 网址 | |
| DOI | |
| 其它 | BACKGROUND:Mesenchymal stromal cells (MSC) have shown diverse therapeutic potential. While characterization of human and mouse MSC has seen significant advances, rat bone marrow-derived MSC (rBM-MSC) remain under-characterized. We detail the isolation, expansion, differentiation, and detailed immunocharacterization of rBM-MSC. METHODS:Rat MSC were isolated and expanded in multipotent adult progenitor cell (MAPC) media, and cell-surface marker expression through 10 passages was used to characterize the population and multipotency was confirmed via differentiation. RESULTS:By passage 3, rBM-MSC were found to be CD11b-, CD45-, CD29+, CD49e+, CD73+, CD90+, CD105+ and Stro-1+, without the use of cell sorting. Media selection was responsible for the isolation of a nearly homogeneous population of rBM-MSC. The rBM-MSC immunophenotype changed by passage 10, showing decreases in CD73, CD105 and Stro-1 expression. |
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